Derivative Assay:Article Title: The ROCK1 PHC1 domain mediates recruitment to Rho activity zones and contractility signaling.
Article Snippet: .. U2OS cells (ATCC, RRID: CVCL_0042), derived from a female donor, were cultured in DMEM + GlutaMAX (Ref.: 31966-047; Gibco) (10% FBS [Ref.: A5256701; Gibco], 10 U/ml penicillin + 100 μg/ml streptomycin [Cat. no.: P06-07100; PAN-Biotech]). .. HEK293T cells (ATCC RRID: CVCL_0063), a derivative of HEK293 cells of reported female fetal origin, were cultured in DMEM (P04-035550; PAN-Biotech) supplemented with 10% FCS + 100 U/ml penicillin and 100 U/ml streptomycin.
Cell Culture:Article Title: The ROCK1 PHC1 domain mediates recruitment to Rho activity zones and contractility signaling.
Article Snippet: .. U2OS cells (ATCC, RRID: CVCL_0042), derived from a female donor, were cultured in DMEM + GlutaMAX (Ref.: 31966-047; Gibco) (10% FBS [Ref.: A5256701; Gibco], 10 U/ml penicillin + 100 μg/ml streptomycin [Cat. no.: P06-07100; PAN-Biotech]). .. HEK293T cells (ATCC RRID: CVCL_0063), a derivative of HEK293 cells of reported female fetal origin, were cultured in DMEM (P04-035550; PAN-Biotech) supplemented with 10% FCS + 100 U/ml penicillin and 100 U/ml streptomycin.
Article Title: TDP-43 amyloid conversion in cells tracks the net charge of a polyampholyte motif, not condensate solidification
Article Snippet: .. U2OS cells (ATCC, HTB-96, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM with high glucose; Cytiva, USA SH30023.01) supplemented with 10% fetal bovine serum (FBS; Cytiva, USA, SH30088.03) and 1X antibiotic-antimycotic solution (Anti-Anti; Gibco, USA, 15240-062) at 37°C in a humidified incubator with 5% CO2. .. Once the cells reached 70% confluence, they were transfected with pcDNA3.1-myc-hisA expression vectors encoding GFP-TDP-43 and its charge-cluster deletion, charge-neutralization and PAM substitution mutants using Lipofectamine 3000 (Invitrogen, USA, L300015), following the manufacturer’s protocol.
Article Title: Exploiting the bacterial type III secretion system of Pseudomonas aeruginosa to impact genomic recombination in murine lungs
Article Snippet: .. U2OS cells (ATCC, Cat# HTB-96) were cultured in DMEM (Corning, Cat# 10–013) supplemented with heat-inactivated FBS (final concentration 10%; R&D Systems, Cat# S11140H); and 100 U/mL penicillin/ streptomycin (Gibco, Cat# 15140163) under standard cell culture conditions (37 ◦C, 5% CO2) in a humidified incubator. .. Genetica Cell Line Testing was used to routinely validate the U2OS cells.
Modification:Article Title: TDP-43 amyloid conversion in cells tracks the net charge of a polyampholyte motif, not condensate solidification
Article Snippet: .. U2OS cells (ATCC, HTB-96, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM with high glucose; Cytiva, USA SH30023.01) supplemented with 10% fetal bovine serum (FBS; Cytiva, USA, SH30088.03) and 1X antibiotic-antimycotic solution (Anti-Anti; Gibco, USA, 15240-062) at 37°C in a humidified incubator with 5% CO2. .. Once the cells reached 70% confluence, they were transfected with pcDNA3.1-myc-hisA expression vectors encoding GFP-TDP-43 and its charge-cluster deletion, charge-neutralization and PAM substitution mutants using Lipofectamine 3000 (Invitrogen, USA, L300015), following the manufacturer’s protocol.
Incubator:Article Title: TDP-43 amyloid conversion in cells tracks the net charge of a polyampholyte motif, not condensate solidification
Article Snippet: .. U2OS cells (ATCC, HTB-96, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM with high glucose; Cytiva, USA SH30023.01) supplemented with 10% fetal bovine serum (FBS; Cytiva, USA, SH30088.03) and 1X antibiotic-antimycotic solution (Anti-Anti; Gibco, USA, 15240-062) at 37°C in a humidified incubator with 5% CO2. .. Once the cells reached 70% confluence, they were transfected with pcDNA3.1-myc-hisA expression vectors encoding GFP-TDP-43 and its charge-cluster deletion, charge-neutralization and PAM substitution mutants using Lipofectamine 3000 (Invitrogen, USA, L300015), following the manufacturer’s protocol.
Article Title: Exploiting the bacterial type III secretion system of Pseudomonas aeruginosa to impact genomic recombination in murine lungs
Article Snippet: .. U2OS cells (ATCC, Cat# HTB-96) were cultured in DMEM (Corning, Cat# 10–013) supplemented with heat-inactivated FBS (final concentration 10%; R&D Systems, Cat# S11140H); and 100 U/mL penicillin/ streptomycin (Gibco, Cat# 15140163) under standard cell culture conditions (37 ◦C, 5% CO2) in a humidified incubator. .. Genetica Cell Line Testing was used to routinely validate the U2OS cells.
Concentration Assay:Article Title: Exploiting the bacterial type III secretion system of Pseudomonas aeruginosa to impact genomic recombination in murine lungs
Article Snippet: .. U2OS cells (ATCC, Cat# HTB-96) were cultured in DMEM (Corning, Cat# 10–013) supplemented with heat-inactivated FBS (final concentration 10%; R&D Systems, Cat# S11140H); and 100 U/mL penicillin/ streptomycin (Gibco, Cat# 15140163) under standard cell culture conditions (37 ◦C, 5% CO2) in a humidified incubator. .. Genetica Cell Line Testing was used to routinely validate the U2OS cells.
other:Article Title: Human pannexin mutations and their implications in erosive osteoarthritis.
Article Snippet: In this collaborative paper, we discovered the first germline mutation in the Pannexin 3 (PANX3) gene that is associated with disease.. This PANX3 mutation results in a loss of channel function that is associated with erosive hand osteoarthritis (EHOA) in humans.. In contrast, a second distinct mutation reported in this study found on Pannexin 1 (PANX1) in a different family of EHOA patients, developed a gain of function mutation thereby marking the first evidence of PANX1 as a genetic factor linked to EHOA.
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![a Pearson correlation scatter plots for compounds sharing the same MoA, comparing <t>U2OS</t> and RPTEC data. Pearson correlation was calculated between morphological profiles of compounds sharing the same MoA. Each dot represents the correlation score for an individual MoA, comparing the U2OS data (y-axis) with the RPTEC data (x-axis). Dotted lines mark the threshold of 0.75, indicating high correlation. Coloured labels indicate the MoAs specifically discussed in the main text. b Correlation between PIK3CA antagonists in RPTEC and U2OS cells. Symbols represent normalised values of single morphological features, corresponding channels, regions and modules are encoded by colour, shape and letter, respectively. r indicates Person correlation coefficient. c Morphological profile heatmap (B-scores and robust z-scores) for PIK3CA antagonists in RPTEC and U2OS. Modules, regions, and channels are colour-coded. A black-outlined box highlights lysosome-related features. (B-scores and z-scores are clipped to the range [-6, +6], values > 6 are set to 6 and values < −6 are set to −6 for display). d Morphological profile heatmap for TOP1/2 antagonists in RPTEC and U2OS. Modules, regions, and channels are colour-coded as indicated in panel c. The black-outlined box highlights DNA-related features. Lyso=lysosomes; Mito=mitochondria; ER=endoplasmic reticulum; Golgi=Golgi apparatus.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_29/10__64898_slash_2026__10__06__756929/10__64898_slash_2026__10__06__756929___F6.large.jpg)
